Immobilized lipolytic enzyme for esterification and interesterif

Details Immobilized lipolytic enzyme for esterification and interesterif Immobilized lipolytic enzyme for esterification and interesterif

1991-04-12

1992-07-07

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435135, 435177, 435180, 435198, 435939, C12P 764, C12P 762, C12N 1108

Patent

active

051282518

ABSTRACT:
An immobilized lipolytic enzyme for esterification and interesterification of fats and oils is prepared by adsorbing a fatty acid or a derivative thereof onto an insoluble carrier and then adsorbing a lipolytic enzyme onto the carrier in an aqueous medium. The resultant immobilized lipolytic enzyme can be dried to a water content of 2 wt. % or less. Preferably, the carrier is a phenol/formaldehyde resin, the lipolytic enzyme is from Rhizopus japonicus and the fatty acid is linoleic acid, lauric acid, stearic acid, ricinoleic acid or isostearic acid. A reaction column can be charged with the dried immobilized lipolytic enzyme and reaction continuously carried out without the use of a solvent. Preferably, starting materials have a water content of 0.01 to 0.2 wt. % and residence time in the column is 2 hours or shorter.

REFERENCES:
patent: 3798793 (1974-01-01), Eigtved
patent: 3909360 (1975-09-01), Horiuchi et al.
patent: 4472503 (1984-09-01), Matsuo et al.
patent: 4818695 (1989-04-01), Eigtved
Chemical Abstracts, vol. 102, No. 11, 1985, p. 470, No. 94351q.
Chemical Abstracts, vol. 107, No. 13, 1987, p. 293, No. 111803m.
"Application of Immobilized Lipase to Hydrolysis of Triaglyceride", Eur. J. App. Microbiol Biotechnol (1983), 17: 107-112.
"Purification and Some Properties of Cell-bound Lipase from Saccharomycopsis lipolytica", Agric. Biol. Chem. 46, (12) pp. 2885-2893, 1982.
"Isolation and Identification of Lipase Activators from Saccharomycopsis lipolytica", Agric. Biol. Chem. 50, (10) pp. 2525-2530, 1986.
"Lipase-Catalyzed Interesterification of Oils and Fats", JAOCS, vol. 60. No. 2, pp. 243A-246A., 1983.

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