Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
1999-05-28
2001-01-09
Russel, Jeffrey E. (Department: 1653)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
C422S028000, C422S032000, C426S310000, C426S322000, C426S326000, C426S332000, C426S335000, C426S532000, C514S008100, C514S021800
Reexamination Certificate
active
06172040
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates to the chemical arts. In particular, it relates to antimicrobial agents and their use.
2. Discussion of the Related Art
Prior to slaughter, the edible tissues of a healthy meat animal are essentially sterile. Various innate host defense mechanisms at the external and internal organ surfaces create an effective barrier and prevent microorganisms from invading a live animal. As soon as the animal is slaughtered, however, the natural defenses against invading microbes virtually disappear, and the exposed tissues become highly susceptible to microbial colonization and proliferation. Meat of the freshly slaughtered animal is prone to contamination with a variety of bacterial species, influenced by the degree of sanitation practiced during the meat processing and packing operations.
The economic impact of food-borne pathogenic outbreaks and the shorter than desired shelf life of refrigerated products, even vacuum packaged refrigerated products, has necessitated the search for an effective antimicrobial system for the meat industry. The recent occurrence of verotoxic
Eschierichia coli
(
E. coli
) serotype O157:H7 in ground beef causing hemolytic uremic syndrome highlighted this longstanding problem, in foods and, especially, meats. It has prompted a major review of safety issues in the food industry and a call for improved methods for preventing microbial contamination. Various methods are currently in practice to control
Eschanchia coli
and other microbial contamination in foods, but, unfortunately, they suffer from a variety of drawbacks.
For example, in the meat industry, acid washing of beef carcasses is currently being employed as a microbial intervention. However, recent studies have shown that certain types of
E. coli
, such as the verotoxic strains of serotype O157:H7 and vancomycin-resistant strains of
Enterococcus faeciumi
, can survive acid conditions, while at the same time produce harmful toxins. The meat industry is also irradiating meat in an attempt to control pathogens and food spoilage organisms. However, studies have shown that although irradiation appears to be effective at killing some types of
E. coli
, there are still various other microorganisms, including strains of
Brochothrix thermospacta
and
Bacillis pumilus
, known to be radiation resistant and thus able to survive such processes. Irradiation also can produce undesirable changes in the texture and/or organoleptic quality of beef. Further, both of these methods are cidal processes that kill microorganisms leaving endotoxins, microbial debris and other proinflammatory substances which can cause undesirable immunological reactions in the host. Finally, neither of these methods excludes the possibility of post-processing contamination once the beef is treated for microbial contaminants.
Under certain conditions, it is possible to control microbes, including
E. coli
, using such well known antimicrobial agents as acids, salts, oxidative agents, antibiotics, bacteriocins, and the like. Typically, the mode of action of these agents is cidal—the direct killing of the microbes— or stasis—the inhibition of microbial growth-multiplication. Another mode of action for conventional antimicrobial agents is opsonization. The agents intervene by promoting microbial phagocytosis by macrophages.
Certain cellular research relating to the mechanisms of microbial biosurface interactions has led to the identification of another mode of action, microbial blocking, and a new class of antimicrobial agents, microbial blocking agents (MBAs). MBAs are naturally occurring biological substances that block microbial adhesion-colonization, retard growth-multiplication, and neutralize the adverse effects of proinflammatory cell debris.
It has not proved possible to apply such microbial blocking agents during meat packing or other food processing conditions, because of the difficulty of delivering a biofunctionally active and structurally stable MBA to the food product to be treated. The difficulty is compounded when the food product is a meat product, because a controlled milieu is required for a broad-spectrum activity of MBA to block various microorganisms on a chemically complex and heterogenous meat tissue.
Lactoferrin (LF) is an iron-bindinig glyco-protein present in milk and various mammalian secretions (e.g. saliva, tears, mucus, and seminal fluids). Crystallographic studies of LF indicate a bilobate structure (N-terminus and C-terminus lobes) with one iron-binding site in each lobe. LF has ability to reversibly bind two Fe
3+
ions per lobe in coordination with two CO
3
2−
ions. LF can release the bound iron in a fully reversible manner, either on exposure to lowered pH (below 4.0) or on receptor binding. This high affinity for iron is linked to many of its biological functions including antimicrobial effects. Various laboratory studies have reported that the structural integrity of LF is critical for its antimicrobial effects against bacteria, fungi, protozoa, and viruses.
However, the activity of LF, like the activity of most proteins, is highly dependent on the three-dimensional or tertiary structure of the protein. If the protein does not have the proper conformation its activity is diminished or lost. LF's instability limits it usefulness. Milieu conditions such as metals (iron in particular), carbonic ions, salts, pH and conductivity affect the antimicrobial properties of LF. In addition, protein isolation procedures, storage, freezing-thawing, can adversely affect the biofunctionality of LF. Consequently, before LF can be used for commercial application, it would be expected to become denatured or inactivated, and lose its antimicrobial properties.
In fact, under certain conditions, when the LF molecule is degraded or denatured, cationic peptide fragments are generated. These cationic peptides exhibit a non-specific antimicrobial activity, making them absolutely unsuitable as an ingredient in a food product. The consumer of a food product does not want to ingest a non-specific antimicrobial agent, because of the agent's adverse affect on the desirable microbes always present in a human body.
Thus, an antimicrobial agent is needed for blocking microbial contamination in foods, and meats in particular, that does not pose the undesired affects of cidal antimicrobial systems but that also exhibits carry through properties for the prevention of post-processing food contamination.
SUMMARY OF THE INVENTION
The present invention relates to a method for treating products with a sufficient amount of lactofeltin to reduce microbial contamination and the immobilized lactofenrin used in the process. More particularly, the present invention relates to immobilized lactofeltin and mixtures of immobilized lactofenrin and native lactoferrin having increased antimicrobial activity against a wide variety of bacteria and other food spoilage as well as increased stability for use in various stages of food processing, and a method for treating foods, and particularly meats, such as beef, pork and poultry products. The method is of use in preventing microbial contamination by a wide variety of microbes including enterotoxigenic
Escherichia coli
, enteropathogenic
Escherichia coli, Shigella dysenteriae, Shigella flexneri, Salmonella typhimurium, Salmonella abony, Salmonella dublin, Salmonella hartford, Salimonella kentucky, Salmonella panama, Salmonella pullorum, Salmonella restock, Salmonella thompson, Salmonella virschow, Campylobacter jejuni, Aeromonas hydrophila, Staphylococcus aureus, Staphylococcus hyicus, Staphylococcuis epidermidis, Staphylococcus hominis, Staphylococcus warneri, Staphylococcus xylosus, Staphylococcus chromogenes, Bacillus cereus, Bacillus subtilis, Candida albicans
, and such radiation-resistant bacteria as:
Brochothrix thermospacta, Bacillus pumilus, Enterococcus faecium, Deinococcus radiopugnans, Deinococcus radiodurans, Deinobacter grandis, Acinetobacter radioresistens, Methylobacterium radiotolerans
. It is
Naidu A. Satyanarayan
Pretty & Schroeder, P.C.
Russel Jeffrey E.
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