Immobilized hydrophobically-modified antibodies

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic

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436527, 5303911, 5303919, 435 71, G01N 33552, G01N 33531, C07K 1528, C07K 1700

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active

053148304

ABSTRACT:
The invention comprises stable, antigen-sensitive surfaces containing immobilized antibody modified with a polyethylene glycol spacer arm including a terminal hydrophobic moiety. The surfaces are generally useful in immunoassay procedures, especially microgravimetric assays such as those employing antibody immobilized on piezoelectric crystal surfaces.

REFERENCES:
patent: 4735906 (1988-04-01), Bastiaans
patent: 4808530 (1989-02-01), Means et al.
patent: 4952519 (1990-08-01), Lau
patent: 5059654 (1991-10-01), Hou et al.
Anderson, et al.; Journal of Immunological Methods, vol. 109, No. 1, pp. 37-42; 1988.
Shons, A. (1972). Immunoassay with coated piezoelectric crystals. J. Biomed. Materl. Res. 6: 565-570.
D'Souza, S. F., Melo J. S., Deshpande, A., and Nadkarni, G. (1986). Immobilization on yeast cells by adhesion to glass surfaces using polyethyleneimine. Biotech. Lett. 8:643-648.
Blanchard, G. C., Taylor, C. G., Busey, B. R., and Williamson, M. L. (1990). Regeneration of immunosorbent surfaces used in chemical, industrial and environmental biosensors. Role of covalent and non-covalent interactions. J. Immunol. Methods 130: 263-275.
Boitieux, J. L., Desmet, G., Wilson G. and Thomas, D. (1990). The specific immobilization of antibody fragments on membrane for the development of multifunctional biosensors. Ann. N.Y. Acad. Sci. 613: 391-395.
Guilbault, G. G., Loung, J. H. T., Prusak-Sochaczewski, E. (1989). Immobilization methods for piezoelectric biosensors. BioTechnology 7: 349-351.
Loung, J. H. T., Prusak-Sochaczewski, E., and Guilbault, G. G. (1990). Development of a piezoimmunosensor for the detection of Salmonella typhimurium. Ann N.Y. Acad. Sci. 613: 439-443.
Muramatsu, H., Dicks, J. M., Tamiya, E. and Karube, I. (1987). Piezoelectric crystal biosensor modified with protein A for determination of immunoglobulins. Anal. Chem. 59: 2760-2763.
Rajakovic, L., Ghaemmaghami, V., and Thompson, M. (1989). Adsorption on film-free and antibody-coated piezoelectric sensors. Anal. Chim. Acta 217: 111-121.
Roederer, J. E. and Bastiass, G. J. (1983). Microgravimetric immunoassay with piezoelectric crystals. Anal. Chem. 55: 2333-2336.
Suleiman, A. A., and Guibault, G. G. (1991). Piezoelectic Immunosensors and their applications. Anal. Lett. 24: 1283-1292.
Sutherland, R. M., Dahne, C., Place, J. F., and Ringrose, A. S. (1984). Optical detection of antigen-antibody reactions at a glass-liquid surface. Clin. Chem. 30: 1533-1538.

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