Immobilized glucoamylase reactor for preparing a low calorie bee

Food or edible material: processes – compositions – and products – Fermentation processes – Alcoholic beverage production or treatment to result in...

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426 16, 435176, 435288, C12C 1104, C12N 1114, C12M 140

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active

044303483

ABSTRACT:
A superattenuated low caloric beer is produced by passing fermenting beer through an immobilized glucoamylase reactor having active glucoamylase immobilized on a ceramic monolith. The ceramic monolith has open ended passages ranging from 50 to 3,000 microns in diameter through which the beer passes, and glucoamylase is covalently bonded to internal surfaces of the passages. The glucoamylase is preferably covalently bonded by reacting an aldehydic derivative of glucoamylase with an aminofunctional group on the internal surfaces of the passages to form an aldimine which is reduced to a secondary amine. The reactor can sustain high flow rates of beer containing yeast and other particulate matter without plugging or fouling, and it is operationally stable under fermentation conditions over a long period of time.

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patent: 4051011 (1977-09-01), Miyauchi et al.
Vieth, et al., Design and Analysis of Immobilized Enzyme Flow Reactors, Applied Biochemistry and Bioengineering, vol. I, 1976, (pp. 221-234).
Wang, et al., Collagan-Enzyme Complex Membranes and Their Performance In Biocatalytic Modules, Biotechnol. & Bioeng., vol. XV, 1973, (pp. 93-115).
Zaborsky, O. R., Immobilized Enzymes-Miscellaneous Methods and General Classification Methods in Enzymology, vol. XLIV, 1976, (pp. 317-332).

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