Immobilized enzymes for use in an electrochemical sensor

Details Immobilized enzymes for use in an electrochemical sensor Immobilized enzymes for use in an electrochemical sensor

1994-07-15

1995-12-19

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an...

435177, 435180, 435181, 435817, 204403, C12N 1114, C12N 1102, C12N 1108, G01N 2726

Patent

active

054767768

ABSTRACT:
A reusable, miniature, implantable electrochemical sensor, a method of making the same, and a powder therefor are provided. Enzyme material is immobilized on bulk particulate matter, and a reaction chamber of the sensor is then filled therewith. The sensor is implanted in an environment where it comes into contact with a specific component of a fluid with which the enzyme material chemically reacts to produce electrical signals for measuring the reaction. The method preferred for preparing the powder which is used in the electrochemical sensor involves first covalently bonding a quantity of an enzyme to fine particles in powder form to immobilize the enzyme is cross-linked to a non-enzyme protein with a cross-linking agent. Finally, the particles are added containing the immobilized enzyme cross-linked to a protein from the first step to the enzyme cross-linked to a protein from the second step to obtain the powder.

REFERENCES:
patent: 3519538 (1970-07-01), Messing et al.
patent: 3928143 (1975-12-01), Coughlin et al.
patent: 4421850 (1983-12-01), Daniels et al.
patent: 4438196 (1984-03-01), Lantero, Jr.
patent: 4464468 (1984-08-01), Avrameas et al.
patent: 4579942 (1986-04-01), Brode et al.
patent: 4986271 (1991-01-01), Wilkins
patent: 5137818 (1992-08-01), Harder et al.
patent: 5156956 (1992-10-01), Motoki et al.
patent: 5314814 (1994-05-01), Harder et al.
Lilly et al., Enzyme Eng., vol. 3, pp. 73-78, 1978.
Turko et al., Biochima. et Biophysica Acta, 996, 1989, 37-42.
Warth et al., Biochimie, 71, 1989, 613-23.
Packman et al., Biochem. J., 1982, 205, 389-96.
Dolla et al., Biochimica et Biophysica Acta 932, 1988, 26-32.

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