Immobilized enzyme membrane

Details Immobilized enzyme membrane Immobilized enzyme membrane

1979-09-28

1981-12-22

Naff, David M.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

204 1T, 435179, 435181, 435182, C12M 140, C12N 1104

Patent

active

043071953

ABSTRACT:
An immobilized enzyme membrane for use at the working face of an electrochemical electrode is prepared which comprises an asymmetrical membrane integrally formed from a skin layer substantially incapable of permeating an enzyme therethrough but capable of permeating a gas and a liquid, and a sponge layer having pores containing an enzyme immobilized therein by crosslinking and which pores intercommunicate with one another throughout the sponge layer and provide sufficient porosity for retaining a necessary amount of the enzyme. The immobilized enzyme membrane contains a large amount of enzyme, has good diffusion and permeability, and has stabilized enzyme activity for a prolonged period of time. Additionally, there is obtained a quick response time and good analytical precision when the immobilized enzyme membrane is used at the working face of an electrode of electrochemical measuring instruments.

REFERENCES:
patent: 3539455 (1970-11-01), Clark, Jr.
patent: 3542662 (1970-11-01), Hicks et al.
patent: 3705084 (1972-12-01), Reynolds
patent: 4033822 (1977-07-01), Gregor
Zaborsky, O., Immobilized Ensymes, CRC Press, Cleveland, Ohio, 1973 (pp. 70-74, 103-115 & 127-133).
Selegny, et al., Enzymatically Active Model-Membranes, Physiol. Veg., vol. 9 No. 1, 1971 (pp. 25-30).

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