Immobilized enzyme having reversible solubility

Details Immobilized enzyme having reversible solubility Immobilized enzyme having reversible solubility

1986-05-08

1988-11-08

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435177, C12N 1108, C12N 1102

Patent

active

047834099

ABSTRACT:
An immobilized enzyme having reversible solubility is prepared by attaching an enzyme to a copolymer of two of more monomers selected from the group consisting of acrylic acid or an alkyl ester thereof, methacrylic acid or alkyl ester or dialkylamino-alkyl ester thereof, and a vinylpyridine derivative of the general formula [U]: ##STR1## wherein R is an alkyl. Preferred capolymers are methacrylic acid-alkyl methacrylate-alkyl acrylate, methacrylic acid-alkyl methacrylate, alkyl acrylate-methacrylic acid, alkyl acrylate-methacrylic acid-alkyl acrylate, alkyl acrylate-methacrylic acid-2-methyl-5-vinylpyridine and dialkylaminoalkyl methacrylate-alkyl methacrylate. The immobilized enzyme has a high activity and can dissolve even in an organic solvent or in a solution containing an organic solvent.

REFERENCES:
patent: 4017364 (1977-04-01), van Leemputten
patent: 4349630 (1982-09-01), Maximenko et al.
Margolin et al., Biotechnology and Bioengineering, vol. XXIV, 1982, pp. 237-240.

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