Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1988-01-20
1990-11-13
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435817, 435 25, 435174, 435291, 435176, 435177, 435680, 435181, 204403, C12M 140, C12N 904
Patent
active
049701452
ABSTRACT:
Enzyme electrodes are disclosed which are capable of responding amperometrically to the catalytic activity of the enzyme in the presence of its respective substrate and comprising the enzyme immobilized or adsorbed onto the surface of an electrically conductive support member which consists of or comprises a porous layer of resin-bonded carbon or graphite particles, said particles having intimately mixed therewith, or deposited or adsorbed onto the surface of the individual particles prior to bonding to form said layer, a finely divided platinum group metal, thereby to form a porous, substrate layer onto which said enzyme is adsorbed or immobilized and comprising a substantially heterogeneous layer of resin-bonded carbon or graphite particles, with said platinum group metal dispersed substantially uniformly throughout said layer. The preferred substrate materials are resin bonded platinized carbon paper electrodes comprising platinized carbon powder particles having colloidal platinum adsorbed on the surface of the particles and bonded onto a carbon paper substrate using a synthetic resin, preferably polytetrafluoroethylene, as the binder. The preferred enzyme electrodes are glucose oxidase electrodes comprising glucose oxidase adsorbed or immobilized onto the surface of the substrate.
REFERENCES:
patent: 4229490 (1980-10-01), Frank et al.
patent: 4415666 (1983-11-01), D'Orazio
patent: 4820399 (1989-04-01), Senda et al.
Aston and Turner, Biosensors and Biofuel Cells, Biotechnology and Genetic Engineering Reviews, 1, Feb. 1984, 89-120.
Graham Davis, Electrochemical Techniques for the Development of Amperometric Biosensors, Biosensors, 1, 161-178 (1985).
Jonsson and Gorton, An Amperometric Glucose Sensor Made by Modification of a Graphite Electrode Surface with Immobilized Glucose Oxidase and Adsorbed Mediator, Biosensors, 1, 355-369 (1985).
Cass et al., Ferrocene--Mediated Enzyme Electrode for Amperometric Determination of Glucose, Analyt. Chem., 56, 667-673 (1984).
Ianiello et al., Differential Pulse Voltammetric Study of Direct Electron Transfer in Glucose Oxidase Chemically Modified Graphite Electrodes, (1982), Analyt. Chem., 54, 1098-1101.
Ianiello and Yacynych, Immobilized Enzyme Chemically Modified Electrode as an Amperometric Sensor, (1981), Analyt. Chem., 53, 2090-2095.
Tarasevich, Bioelectrochemistry 10, 231-295 (1985).
Bennetto Hugh P.
DeKeyzer David R.
Delaney Gerard M.
Mason Jeremy R.
Stirling John L.
Cambridge Life Sciences PLC
Waack Janelle D.
Wax Robert A.
LandOfFree
Immobilized enzyme electrodes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immobilized enzyme electrodes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immobilized enzyme electrodes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-776510