Immobilized DNA polymerase

Details Immobilized DNA polymerase Immobilized DNA polymerase

2007-07-03

2007-07-03

Naff, David M. (Department: 1657)

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

C435S006120, C435S177000, C435S178000, C536S022100

Reexamination Certificate

active

10406154

ABSTRACT:
The present invention relates to a DNA polymerase immobilized by covalent bonding. More particularly, the present invention relates to an immobilized DNA polymerase whose activity is maximally preserved by masking the active site of the DNA polymerase and optimizing interaction of the masked molecule to the substrate material. In one embodiment, the average activity of the immobilized DNA polymerase is more than about 10% relative to that of the solution phase DNA polymerase. Further provided by the invention are methods and kits for performing polymerase chain reactions (PCR).

REFERENCES:
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patent: 6194552 (2001-02-01), Velander et al.
patent: 6610479 (2003-08-01), Lundeberg et al.
patent: WO 94/13322 (1994-06-01), None
patent: WO 02/04483 (2002-01-01), None
patent: WO 02/29027 (2002-04-01), None
Subramanian, et al., “Effect of Antibody Orientation on Immunosorbent Performance”; Journal of Molecular Recognition, vol. 9, pp. 528-535, 1996.

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