Immobilized cells for preparing phenylalanine

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

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435180, 435182, C12P 1322, C12N 1108, C12N 1104

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active

046006923

ABSTRACT:
A process is disclosed for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having transaminase activity in the presence of an amine donor. The cells are preferably immobilized with a polyazetidine polymer. Ruptured or permeabilized cells, with the enzyme in the free or immobilized state, may also be used. The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also disclosed.

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Oishi, ("The Microbial Production of Amino Acids", John Wiley & Sons, K. Yamada et al., Eds. 1972, Chap. 16).
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