Food or edible material: processes – compositions – and products – Dormant ferment containing product – or live microorganism... – Yeast containing
Patent
1997-03-10
1999-02-09
Sherrer, Curtis E.
Food or edible material: processes, compositions, and products
Dormant ferment containing product, or live microorganism...
Yeast containing
426 16, 435177, 435182, C12N 1102, C12N 1104
Patent
active
058691170
ABSTRACT:
A process for immobilizing viable cells in gelled carrageenan beads comprises preparing an aqueous phase that is a mixture of a gellable concentration of un-gelled carrageenan, in an aqueous suspension of viable cells, in which the mixture's potassium concentration is low enough that the thermogellation temperature of the carrageenan in the suspension is below a temperature to which the viable cells are substantially thermosensitive. This is done at a first processing temperature that exceeds the thermogellation temperature of the carrageenan in that aqueous suspension, but which is below the temperature to which the cells are substantially thermosensitive. A mixture of the aqueous phase and a non-reactive food-grade oil phase is then prepared, and subjected to shear by passing it through a static mixer under flow-rate conditions selected to disperse the aqueous phase in the oil phase, such that a resulting emulsion has a selected droplet size distribution. Thereafter, the temperature of the resulting emulsion is decreased from the first processing temperature, to a second processing temperature which is below the thermogellation temperature of the carrageenan in the suspension. This results in the formation of polymer gel beads having the immobilized viable cells entrapped therein.
REFERENCES:
patent: 4647536 (1987-03-01), Mosbach et al.
patent: 4698224 (1987-10-01), Nakanishi et al.
patent: 4940734 (1990-07-01), Ley et al.
patent: 5037740 (1991-08-01), Tanaka et al.
patent: 5070019 (1991-12-01), Hill
patent: 5079011 (1992-01-01), Lommi et al.
patent: 5662840 (1997-09-01), Thomas et al.
Nagashima, et al., "Large-Scale Preparation of Calcium Alginate-Immobilized Yeast Cells and Its Application to Industrial Ethanol Production," Methods in Enzymology vol. 136 (1987), 394-405.
Nojima, et al., "Large-Scale Production of Photo-Cross-Linkable Resin-Immobilized Yeast and Its Application to Industrial Ethanol Production," Methods in Enzymology vol. 136 (1987), 380-394.
Tosa, et al., "Immobilization of Enzymes and Microbial Cells Using Carrageenan as Matrix," Biotechnology and Bioengineering vol. XXI (1979), 1697-1709.
Ogbonna, et al., "Production of Micro-Gel Beads by a Rotating Disk Atomizer," Journal of Fermentation and Bio-engineering vol. 68, No. 1 (1989), 40-48.
Nakanishi, et al., "A new immobilized yeast reactor system for rapid production of beer," EBC Congress (1985) 331-338.
Nakanishi, et al., "A New Immobilized Yeast Reactor System for Rapid Production of Beer," Rep. Res. Lab. Kirin Brew Co. No. 29 (1986), 13-16.
Aivasidis, et al., "Continuous fermentation of alcohol-free beer with immobilized yeast in fluidized bed reactors," Proc. Eur. Brew. (1991) 569-576.
Dr. Klaus Breitenbucher, "Continuous Brewing with Immobilized Brewing Yeast," Brew. Beverage Ind. Inl. (1992), 28, 30 and 31.
Godia, et al., "Immobilized Cells; Behavior of Carrageenan Entrapped Yeast during Continuous Ethanol Fermentation," Appl. Microbiol. Biotechnol. (1987), 342-346.
J.H.T. Luong, "Cell Immobilization in K-Carrageenan for Ethanol Production," Biotechnology and Bioengineering vol. XXVII (1985), 1652-1661.
Nagashima, et al., "Continuous Ethanol Fermentation Using Immobilized Yeast Cells," Biotechnology and Bioengineering vol. XXVI (1984), 992-997.
Takata, et al., "Screening of Matrix Suitable for Immobilization of Microbial Cells," Journal of Solid-Phase Biochemistry vol. 2, No. 3, (1977) 225-236.
Nilsson, et al., "Entrapment of Microbial and Plant Cells in Beaded Polymers," Methods in Enzymology vol. 135 (1987), 222-223.
Audet, et al., "Two-phase Dispersion Process for the Production of Biopolymer Gel Beads: Effect of Various Parameters on Bead Size and Their Distribution," Biochemistry (Dec. 1989), 217-226.
Poncelet, et al., "Production of alginate beads by emulsification/internal gelation: I. Methodology," Applied Microbiology and Biotechnology (1992), 39-45.
Jukka Kronl.o slashed.f, "Immobilized yeast in continuous fermentation of beer," VTT Publications 167 (1994), 1-V/8.
Neufeld Ronald James
Norton Sylvain D. J. M.
Poncelet Denis J. C. M.
Labatt Brewing Company Limited
Sherrer Curtis E.
LandOfFree
Immobilized-cell carrageenan bead production and a brewing proce does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immobilized-cell carrageenan bead production and a brewing proce, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immobilized-cell carrageenan bead production and a brewing proce will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1946928