Immobilized biomolecules and method of making same

Details Immobilized biomolecules and method of making same Immobilized biomolecules and method of making same

1989-05-04

1991-07-23

Lieberman, Allan M.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Compositions to be polymerized by wave energy wherein said...

204165, 204166, 435179, 435180, 435182, 522 87, 524704, 524733, 524744, 5262381, 527201, 527314, C08J 328, C12N 1100, C12N 1112

Patent

active

050344285

ABSTRACT:
A method of biomolecule immobilization is described in which the biomolecule itself or, alternatively, a monomer-conjugated biomolecule, is grafted with free monomer onto a hydrophilic, solid-phase, polymeric substrate which has been pre-irradiated with ionizing radiation. The pre-irradiation step is carried out, preferably at -78.degree. C. in air, while the grafting step is carried out at 0.degree. C. in a substantially oxygen-free atmosphere. The technique is applicable to immobilization of a wide variety of biomolecules, such as enzymes, catalysts, hormones, lectins, drugs, vitamins, antibodies, antigens, nucleic acids, DNA and RNA segments, pesticides, dyes and fertilizers. The products may be used for therapeutic or diagnostic applications or bioseparations.

REFERENCES:
patent: 3925157 (1975-12-01), Hamsher
patent: 3959078 (1976-05-01), Guire
patent: 3989454 (1976-11-01), Arthur, Jr. et al.
patent: 4193845 (1980-03-01), Kaetsu et al.
patent: 4194066 (1980-03-01), Kaetsu et al.
patent: 4272617 (1981-06-01), Kaetsu et al.
patent: 4310397 (1982-01-01), Kaetsu et al.
patent: 4379038 (1983-04-01), Kaetsu et al.
patent: 4436813 (1984-03-01), Wood et al.
patent: 4451568 (1984-05-01), Schneider et al.
D. Campbell et al., "The Preparation and Characterization of Some Cellulose Graft Copolymers v. ESR Study of Preirradiation Grafting of Styrene to Cellulose Acetate," J. Polymer Sci., 7, pp. 429-437(1969).
S. Dilli et al., "Radiation-Induced Reactions with Cellulose," Aust. J. Chem., 24, pp. 981-987 (1971).
K. D. N. Lawrence et al., "The Graft Polymerization of Acrylamide Onto Paper Preirradiated with High Energy Electrons," J. Appl. Polymer Sci., 17, pp. 2653-2666 (1973).
K. Matsuzaki et al., "Radiation-Induced Graft Copolymerization of Mixtures of Styrene and n-Butyl Acrylate Onto Cellulose and Cellulose Triacetate," J. Appl. Polymer Sci., 16, pp. 1339-1355 (1972).
S. Munari et al., "Molecular Weights of Grafted Polystyrene Onto .gamma.-Preirradiated Cellulose Diacetate," J. Appl. Polymer Sci., 14, pp. 807-816 (1970),.
F. S. Radi et al., "Radiation Grafting of Vinyl Monomers Onto Wood Pulp Cellulose II," J. Appl. Polymer Sci., 16, pp. 2685-2696 (1972).
M. U. Sadykov et al., "Radiation-Induced Graft Copolymerization of Vinyl Monomers Onto Cellulose," Proc. Tihany Symp. Radiation Chem., Iobo, ed., pp. 1037-1048 (1971).
T. Yasukawa et al., "Kinetics of Radiation-Induced Grafting Reactions, II. Cellulose Acetate-Styrene Systems," J. Polymer Sci., pp. 2547-2556 (1973).

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