Immobilized biomolecules and method of making same

Details Immobilized biomolecules and method of making same Immobilized biomolecules and method of making same

1986-06-19

1989-05-09

Lieberman, Allan

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Compositions to be polymerized by wave energy wherein said...

204165, 204166, 435179, 435180, 435182, 522 87, 524704, 524733, 524734, 5262381, 527201, 527314, C08J 328, C12N 1100, C12N 1112

Patent

active

048290980

ABSTRACT:
A method of biomolecule immobilization is described in which the biomolecule itself or, alternatively, a monomer-conjugated biomolecule, is grafted with free monomer onto a hydrophilic, solid-phase, polymeric substrate which has been pre-irradiated with ionizing radiation. The pre-irradiation step is carried out, preferably at -78.degree. C. in air, while the grafting step is carried out at 0.degree. C. in a substantially oxygen-free atmosphere. The technique is applicable to immobilization of a wide variety of biomolecules, such as enzymes, catalysts, hormones, lectins, drugs, vitamins, antibodies, antigens, nucleic acids, DNA and RNA segments, pesticides, dyes and fertilizers. The products may be used for therapeutic or diagnostic applications or bioseparations.

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