Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1980-03-13
1983-10-04
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 34, 435177, 435179, 435180, 436528, 436530, 436809, 436811, G01N 3354, C12Q 104, C12N 1102
Patent
active
044079435
ABSTRACT:
An antigen or antibody and an immunochemically neutral protein are immobilized in two stages on a microporous membrane coated with a protein such as zein or collagen. Immobilized antigen to Toxoplasma gondii is used in an immunoassay to detect the presence of antibody to Toxoplasma gondii in serum. Antibody that becomes bound to the immobilized antigen is detected with an antibody- enzyme conjugate. Immobilizing the immunochemically netural protein on the membrane in a second stage immobilization step minimizes nonspecific binding during the immunoassay.
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Toussaint A. J., Improvement of the Soluble Fluoresant-Antibody Procedure, Experimental Parasitology, vol. 19, 1966, (pp. 71-76).
Toussaint et al. Soluble Antigen Fluorescent-Antibody Technique, Applied Microbiology, vol. 13, No. 4, 1965 (pp. 552-558).
Cole Francis X.
Van Voorhis Deborah M.
Cook Paul J.
Millipore Corporation
Naff David M.
Prashker David
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