Immobilized aminoacylase enzyme

Details Immobilized aminoacylase enzyme Immobilized aminoacylase enzyme

1984-11-27

1986-08-26

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435106, 435177, 435181, 435227, 435280, C12N 1108, C12N 1106, C12N 978, C07P 4100

Patent

active

046083405

ABSTRACT:
Immobilized aminoacylase having high specific activity is obtained by covalent bonding of aminoacylase to a partially hydrolyzed Akrilex P type acrylamide-N,N.sup.1 -methylene-bis (acrylamide) copolymer. Covalent bonding is carried out by partially hydrolyzing the copolymer with a base or an acid to form carboxy groups, activating the carboxy groups with a carbodiimide and coupling aminoacylase to the activated carboxy groups. The aminoacylase may be isolated from mammal kidneys by forming an aqueous kidney extract, heat treating the extract and isolating aminoacylase from the heat treated extract.

REFERENCES:
patent: 3964973 (1976-06-01), Hradil et al.
patent: 3970597 (1976-07-01), Sokolovsky et al.
Ohno et al., Polyacrylamide Derivatives of Amino Acid Acylase and Trypsin, Macromolecules, vol. 4, No. 3, 1971, (pp. 350-354).
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, (pp. 24-27).
Chibata, I., Immobilized Enzymes, John Wiley & Sons, N.Y., 1978, (pp. 31-33).

Affiliated with

Also associated with

Rating

Immobilized aminoacylase enzyme does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immobilized aminoacylase enzyme, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immobilized aminoacylase enzyme will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2312471