Immobilization of proteins on inorganic supports

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...

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435190, C07G 700, C07G 702

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active

042062861

ABSTRACT:
A method of producing an immobilized protein composite, i.e. enzymes such as glucose oxidase, on an alumina support, comprising modifying some of the free functional groups of the protein such as amino groups, by reaction with an acid or anhydride, depositing the modified protein on a porous inorganic support at controlled pH and temperature, and cross-linking remaining free functional groups of the protein with adjacent deposited protein functional groups to form interprotein peptide bonds. The resultant composite has long-term continuous use.

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patent: 3804719 (1974-04-01), Messing
patent: 3850751 (1974-11-01), Messing
patent: 3873426 (1975-03-01), Katchalski et al.
patent: 3930951 (1976-01-01), Messing
patent: 3969287 (1976-07-01), Jaworek et al.
patent: 3982997 (1976-09-01), Eaton et al.
patent: 4004979 (1977-01-01), Arrameas et al.
Martensson, K., Preparation of an Immobilized Two-Enzyme System, B-Amylase-Pullulanase to an Acrylic Copolymer for the Conversion of Starch to Maltose, Biotechnol, and Bioeng. vol. XVI, 1974 (pp. 567-577).

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