Immobilization of proteins and peptides on insoluble supports

Details Immobilization of proteins and peptides on insoluble supports Immobilization of proteins and peptides on insoluble supports

1989-07-26

1991-12-10

Page, Thurman K.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

521 53, 530812, 530815, 530817, 435182, C08G 6391, C12N 1104

Patent

active

050719092

ABSTRACT:
The invention pertains to a method for immobilizing proteins or peptides onto a flat, microporous membrane surface in a form suitable for sequence analysis or other chemical or enzymatic processes. The process involves the formation of a thin polymer network that entraps the protein or peptide therein.

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patent: 4774178 (1988-09-01), Egerer et al.
Sequence from Picomole Quantities of Proteins Electroblotted onto Polyvinylidene Difluoride Membranes, Matsudaira JBC. 262, 10035 (1987).
Pappin, D. J. C. et al., Anal. Biochem. 187:10-19 (1990).
Tarr, G. E., J. Protein Chem. 7:293 (1988).
Aebersold, R. et al., J. Biol. Chem. 261:4229 (1986).
Coull, J. et al., in Methods in Protein Sequencing Analysis (1989), Wittman-Liebold, B. (Ed) Splinger-Verlag, Berlin.
Pappin, D. J. C. et al., Abstract Protein Society Meeting, Seattle, Wash., Jul. 29, 1989.

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