Immobilization of nucleic acids on derivatized nylon supports

Details Immobilization of nucleic acids on derivatized nylon supports Immobilization of nucleic acids on derivatized nylon supports

1985-09-30

1989-02-21

Warren, Charles F.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435803, 935 78, C07H 2100, C12Q 168

Patent

active

048065463

ABSTRACT:
Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon having amide groups that have been derivatized to amidine residues. Derivatization of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by reaction with an amine. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample.

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Bio-Rad Bulletin 1110, Bio-Rad Laboratories, 1986, pp. 1-8.

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