Immobilization of nonanchorage-dependent cells

Details Immobilization of nonanchorage-dependent cells Immobilization of nonanchorage-dependent cells

1986-04-28

1990-08-28

Warren, Charles F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435243, 435180, 43524026, 435942, C12N 502, C12N 100

Patent

active

049525069

ABSTRACT:
Nonanchorage-dependent cells are immobilized for cell culturing or the production of cell products, by contracting an aqueous suspension of the cells with a floc formed by the combination of positively and negatively charged ion exchange resins having particle sizes ranging from about 0.01 to about 1.5 microns. The flocs may be formed either prior to contract with the cells or may be formed in situ in the cell suspension upon separate addition of the resins, preferably by first adding the positively charged resin followed by the negatively charged resin. The resin floc operates as a microcarrier for the cells.

REFERENCES:
patent: Re29130 (1977-02-01), Lee et al.
patent: 4036693 (1977-10-01), Levine et al.
patent: 4347320 (1982-08-01), Borglum
patent: 4380590 (1983-04-01), Chong

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