Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1990-08-08
1992-01-21
Doll, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
435174, 436527, 436532, 525 541, C07K 1714, C12N 1114
Patent
active
050829297
ABSTRACT:
A method for attachment of glycocompounds and glycoconjugates to hydrazide gels, wherein a solution of glycocompound or glycoconjugate containing a suitable oxidizing agent is brought into contact with the hydrazide gel without prior removal of the oxidizing agent. Suitably, the solution is introduced into a cartridge containing the hydrazide gel. In accordance with a first embodiment, this method requires relatively short incubations of about 30 minutes for oxidation of the glycosaccharide moiety of the glycocompound or glycoconjugate, followed by about 15 minutes for binding the oxidized glycocompound or glycoconjugate to the hydrazide gel. Pursuant to another embodiment, incubation with the oxidizing agent is carried out simultaneously with bringing the solution into contact with the gel (e.g., within the cartridge). Synthetic polymer gels with terminal hydrazide groups are particularly suitable for use in this method, as these gels are especially resistant to oxidizing agents such as sodium periodate.
REFERENCES:
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patent: 4801687 (1989-01-01), Ngo
patent: 4874813 (1989-10-01), O'Shannessy
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O'Shannessy et al. (1990), Anal. Biochem. 191:1-8.
Cress et al. (1989), Am. Biotech. Lab. 7(2):16-19.
Pierce, Immunotechnology Catalog, pp. B10, Product No. 44900B (1990).
Fung Gilbert
Ngo That T.
BioProbe International, Inc.
Doll John
Kim Kay
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