Immobilization of glucose isomerase

Details Immobilization of glucose isomerase Immobilization of glucose isomerase

1976-04-23

1980-06-17

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 94, C07G 702, C12D 1304

Patent

active

042084822

ABSTRACT:
Immobilized glucose isomerase is prepared by mixing 0.5 to 1.5 parts by weight whole microbial cells containing glucose isomerase with one part by weight agar, combining the resultant mixture with an organic solvent, recovering discrete particles of agar gel with the whole microbial cells entrapped therein and drying the agar gel particles. The immobilized glucose isomerase has long half life stability when used in a column in a continuous process to convert glucose to fructose.

REFERENCES:
patent: 3779869 (1973-12-01), Zienty
patent: 3834988 (1974-09-01), Shieh et al.
patent: 3843442 (1974-10-01), Moskowitz
patent: 3956065 (1976-05-01), Idaszak et al.
Toda et al., Sucrose Inversion by Immobilized Yeast Cells in a Complete Mixing Reactor Biotechnology and Bioengineering, vol. XVII 1975, (pp. 481-497).
Strandberg et al., Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes, Applied Microbiology, vol. 21, No. 4, 1971 (pp. 588-593).
Nilsson et al., The Use of Bead Polymerization of Acrylic Monomers for Immobilization of Enzymes Biochimica et. Biophysics Actz, vol. 268, 1972 (pp. 253-256).

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