Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1998-12-11
2000-12-05
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435176, 435180, C12P 764, C12N 1114, C12N 1108
Patent
active
061565482
ABSTRACT:
An immobilized enzyme preparation for use in an organic medium essentially devoid of free water is prepared using a fluidized bed. An enzyme-containing liquid medium is contacted with a particulate porous carrier which preferably has a particle size of 200-1000 .mu.m and a surface area of 20-1000 m.sup.2 /g, and volatile components of the liquid medium are removed to fix or adsorb the enzyme on the carrier. The carrier may have a hydrophilic surface and an amount of liquid medium is used to prevent agglomeration of the carrier. The enzyme can be adsorbed on a carrier having a hydrophobic surface, and the addition of a hygroscopic substance suppresses agglomeration of the carrier by absorbing excess liquid. The hygroscopic substance may be removed during the removal of volatile components. Contacting of the enzyme-containing liquid and carrier is in a fluidized bed where immobilization and removing volatile components are conducted simultaneously, or contacting is in mixer followed by removing volatile components in a fluidized bed. The enzyme-containing liquid may be atomized onto the carrier in the fluidized bed or in the mixer. Enzymes immobilized include lipase, and the immobilized lipase is used in a trans-esterification reaction.
REFERENCES:
patent: 4689297 (1987-08-01), Good et al.
patent: 5814501 (1998-09-01), Becker et al.
patent: 5879920 (1999-03-01), Dale et al.
Christensen Morten Wurtz
Kirk Ole
Pedersen Christian
Lambiris Elias J.
Naff David M.
Novo Nordisk A S
Rozek Carol E.
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