Immobilization of enzymes

Details Immobilization of enzymes Immobilization of enzymes

1981-02-24

1983-12-20

Nafe, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

435 96, 435174, 435177, C12P 100, C12P 1920, C12N 1100, C12N 1102

Patent

active

044218508

ABSTRACT:
An immobilized enzyme product is produced by contacting an inert particulate support with an aqueous enzyme solution containing preferably at least 25% dissolved solids and with a water miscible organic solvent, and crosslinking the enzyme to insolubilize the enzyme on the support as a gel containing 50-90% water. The water miscible organic solvent is in substantial excess of the amount of water mixed with the support in the process. The solvent may be contacted with the support before or after contacting with the enzyme solution. The resultant immobilized enzyme product has a bulk volume 5 to 300% greater than the bulk volume of the support material. A least a portion of the gel is external to the support and constitutes at least 3% by volume of the immobilized enzyme product.

REFERENCES:
patent: 3796634 (1974-03-01), Haynes et al.
patent: 3980521 (1976-09-01), Amotz et al.
patent: 4004979 (1977-01-01), Avrameas et al.
patent: 4008126 (1977-02-01), Keyes
patent: 4266029 (1981-05-01), Branner-Jorgensen
patent: 4268423 (1981-05-01), Rohrbach et al.
Zaborsky, O. R., Covalent Linkage: III Immobilization of Enzymes by Intermolecular Cross-Linking, Biomedical Applications of Immobilized Enzymes and Proteins, vol. 1, 1977 (pp. 25-35).

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