Immobilization of enzyme on a fibrous matrix

Details Immobilization of enzyme on a fibrous matrix Immobilization of enzyme on a fibrous matrix

2006-12-04

2010-10-12

Lilling, Herbert J. (Department: 1657)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S105000, C435S175000, C435S176000, C435S177000, C435S182000, C435S289100

Reexamination Certificate

active

07811796

ABSTRACT:
A multilayer enzyme immobilization process is provided comprising adsorbing a polyethyleneimine (PEI) solution in a fibrous matrix, and adding an enzyme to the fibrous matrix, which comprises a plurality of fibrils. The process further comprises forming at least two layers of PEI-enzyme aggregates on the fibrils, and cross-linking the multilayer PEI-enzyme aggregates. The process can further comprise washing the fibrils containing the cross-linked PEI-enzyme aggregates with distilled water and acetic acid buffer subsequent to cross-linking. However, the PEI-containing matrix is not washed prior to the addition of enzyme. The enzyme can be β-galactosidase and the fibrous matrix can be cotton cloth. The multilayer immobilized enzyme can be employed in a biocatalyst reactor for production of galacto-oligosaccharides from lactose and the hydrolysis of lactose to glucose and galactose.

REFERENCES:
patent: 4251631 (1981-02-01), Simon
patent: 4572897 (1986-02-01), Amotz et al.
patent: RE33441 (1990-11-01), Wumpelmann et al.
patent: 5057421 (1991-10-01), Hofmann et al.
patent: 5412087 (1995-05-01), McGall et al.
patent: 5472861 (1995-12-01), Lantero et al.
patent: 5541097 (1996-07-01), Lantero et al.
patent: 5563056 (1996-10-01), Swan et al.
patent: 5759578 (1998-06-01), Soon-Shiong et al.
patent: 5939294 (1999-08-01), Sarkki et al.
Talabardon et al., “Acetic Acid Production from Lactose by an Anaerobic Thermophilic Coculture Immobilized in a Fibrous-Ben Bioreactor”, Biotechnol. Prog 2000, 16, pp. 1008-1017.
Huang et al., “Acetate Production from Whey Lactose Using Co-Immobilized Cells of Homolactic and Homoacetic Bacteria in a Fibrous-Bed Bioreactor”, Biotechnology and Bioengineering, vol. 60, No. 4, Nov. 20, 1998, pp. 498-507.
Yang et al., “Production of Cell-Free Xanthan Fermentation Broth by Cell Adsorption on Fibers”, Biotechnol. Prog. 1998, 14, pp. 259-264.
Yang et al., “A Novel Recycle Batch Immobilized Cell Bioreactor for Propionate Production from Whey Lactose”, Biotechnology and Bioengineering, vol. 45, (1995), pp. 379-386.
Albayrak et al., “Production of Galacto-Oligosaccharides From Lactose byAspergillus oryzaeβ -Galactosidase Immobilized on Cotton Cloth”, Biotechnology and Bioengineering, vol. 77, No. 1, Jan. 5, 2002, pp. 8-19.
Zemek et al., “Crosslinked polyethylenimine: and enzyme carrier with spacers of various lengths introduced in crosslinking reaction”, Enzyme Microb. Technol., 1982, vol. 4, Jul., pp. 233-238.
Jendrisak et al., “The Use of Polyethyleneimine in Protein Purification”, Protein Purification: Micro to Macro, Proceedings of a Cetus-UCLA Symposium Held at Frisco, Colorado, Mar. 29-Apr. 4, 1987, pp. 75-97.
Wasserman et al., “High-Yield Method for Immobilization of Enzymes”, Biotechnology and Bioengineering, vol. XXII, (1980), pp. 271-287.
Emneus et al., “Comparison between different inorganic supports for the immobilization of amyloglucosidase and α -amylase to be used in enzyme reactors in flow-injection systems,” Analytica Chimica Acta, 276 (1993), pp. 319-328.
Yang et al., “A Dynamic Light Scattering Study of β -Galactosidase: Environmental Effects on Protein Conformation and Enzyme Activity”, Biotechnol. Prog. 1994, 10, pp. 525-531.
Kumar et al., “Whole blood glucose determination using glucose oxidase immobilized on cotton cheese cloth”, Analytica Chimica Acta 338 (1997), pp. 135-140.
Kamath et al., “Urease Immobilized on Polyethyeneimine Cotton Cloth”, Applied Biochemistry and Biotechnology, vol. 19, 1998, pp. 251-258.
Das et al., “Short Communication: Immobilization of urease from pigeonpea (Cajanus cajanL.) on flannel cloth using polyethyleneimine”, World Journal of Microbiology & Biotechnology, vol. 14, 1998, pp. 927-929.
Yamazaki et al., “Immobilization of Invertase on Polyethylenimine-Coated Cotton Cloth”, Biotechnology Letters, vol. 6, No. 3, (1984), pp. 165-170.
D'Souza et al., “Cloth Bioreactor containing yeast cells immobilized on cotton cloth using polyethyenimine”, Appl Microbiol Biotechnol (1988), 29, pp. 136-140.
Sako et al., “Recent progress on research and applications of non-digestible galacto-oligosaccharides”, International Dairy Journal 9 (1999), pp. 69-80.
Playne et al., “1. Commercially Available Oligosaccharides”, Bulletin of the IDF 313, pp. 10-22, 1996.
Shin et al., “Continuous production of galacto-oligosaccharides from lactose byBullera singularisβ -galactosidase immobilized in chitosan beads”, Process Biochemistry, vol. 33, No. 8, 1998, pp. 787-792.
Foda et al., “Continuous production of oligosaccharides from whey using a membrane reactor”, Process Biochemistry 35 (2000), pp. 581-587.
Yang et al., “Novel Products and New Technologies for Use of a Familiar Carbohydrate, Milk Lactose”, Journal of Dairy Science, vol. 78, No. 11, 1995, pp. 2541-2562.
Berger et al., “Oligosaccharides Synthesis by Free and Immobilized α-Galactosidases Fromthermus aquaticusYT-1”, Biotechnology Letters, vol. 17, No. 10 (Oct. 1995), pp. 1077-1080.
Mozaffar et al., “Effect of glutaraldehyde on oligosaccharide production by β -galactosidase fromBacillus circulans”, Appl Microbiol Biotechnol (1987) 25, pp. 426-429.
Matsumoto et al., “Production of Galactooligosaccharides with β-Galactosidase”, Denpun Kagaku, vol. 36, No. 2, (1989), pp. 123-130.
Mozaffar et al., “Continuous production of galacto-oligosaccharides from lactose using immobilized β—galactosidase fromBacillus circulans”, Appl Microbiol Biotechnol (1986) 25, pp. 224-228.
Sheu et al., “Production of galacto-oligosaccharides by β-galactosidase immobilized on glutaraldehyde-treated chitosan beads” Biotechnology Techniques, vol. 12, No. 4, Apr. 1998, pp. 273-276.
Kminkova et al., “Immobilization of Mold Beat-Galactosidase”, Collection Czechoslovak Chem. Commun. (vol. 53), (1988), pp. 3214-3219.
Berger et al., “Immobilization of β-Galactosidases FromThermus aquaticusYT-1 for Oligosaccharides Synthesis”, Biotechnology Techniques, vol. 9, No. 8, (Aug. 1995), pp. 601-606.
Prenosil et al., “Formation of Oligosaccharides during Enzymatic Lactose: Part I: State of Art”, Biotechnology and Bioengineering, vol. 30, (1987), pp. 1019-1025.
Pedersen et al., “Enzyme Adsorption in Porous Supports: Local Thermodynamic Equilibrium Model”, Biotechnology and Bioengineering, vol. XXVII, (1985), pp. 961-971.
Dissing et al., “Polyelectrolyte complexes as vehicles for affinity precipitation of proteins”, Journal of Biotechnology, 52, (1996), pp. 1-10.
Prenosil et al., “Formation of Oligosaccharides during Enzymatic Lactose Hydrolysis and Their Importance in a Whey Hydrolysis Process: Part II: Experimental”, Biotechnology and Bioengineering, vol. 30, (1987), pp. 1026-1031.
Rugh, “A Comparison of the Formation of Intermediary Products During Lactose Hydrolysis with Free and Immobilized Thermophilic Lactase”, Applied Biochemistry and Biotechnology 7 (1982), pp. 27-29.
Prenosil et al., “Scale-Up of Membrane Fixed Enzyme Reactors: Modelling and Experiments”, Desalination, 53 (1985), pp. 265-278.
Boon et al., “Effect of temperature and enzyme origin on the enzymatic synthesis of oligosaccharides”, Enzyme and Microbial Technology 26 (2000), pp. 271-281.
Onda et al., “Activity and Stability of Glucose Oxidase in Molecular Films Assembled Alternately with Polyions”, Journal of Bioscience and Bioengineering, vol. 87, No. 1, 1999, pp. 69-75.
Dekker, Immobilization of a Lactase onto a Magnetic Support by Covalent Attachment to Polyethyleneimine-Glutaraldehyde-Activated Magnetite, Applied Biochemistry and Biotechnology, vol. 22, 1989, pp. 289-310.
Bardeletti, “Enzyme

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