Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1988-12-23
1991-12-17
Naff, David M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435178, 435182, 43524025, 4352521, 435254, 435288, 435313, C12N 500, C12N 1110, C12N 502, C12M 140
Patent
active
050734911
ABSTRACT:
Method and apparatus are provided for immobilizing and growing cells in airlift bioreactors to obtain increased cell density. Cells are immobilized by forming alginate beads containing cells and gelatin particles, and dissolving the gelatin by heating to form cavities in the beads entrapping the cells. In a growth chamber of an airlift bioreactor, introduced oxygen-containing gas circulates growth medium in contact with the beads resulting in oxygen transfer to cells in the cavities of the beads where growth of the cells occurs. Preferably, bead formation is carried out in the growth chamber by dripping an alginate-cell gelatin suspension into a calcium solution contained in the growth chamber. Growth medium is then supplied to the chamber, and oxygen-containing gas is introduced to result in circulation of the growth medium and growth of the cells.
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Gould George M.
Hoffman-La Roche Inc.
Johnston George W.
Leon Bernard S.
Naff David M.
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