Immobilization of biomolecules on perfluorocarbon surfaces

Details Immobilization of biomolecules on perfluorocarbon surfaces Immobilization of biomolecules on perfluorocarbon surfaces

1991-10-24

1993-12-14

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435180, 436531, 436532, 530815, 530816, C12N 1106, C12N 1108, G01N 33549, G01N 33545

Patent

active

052701930

ABSTRACT:
Biomolecules such as a ligand or binder for the ligand are securely but reversibly attached to a perfluorocarbon carrier with a water soluble polymer, a perfluorocarbon anchoring group and optionally a linker group. The order of steps for carrying out the attachment can vary. For example, the biomolecule is covalently attached to the polymer followed by covalently attaching the anchoring group and attaching the resultant product to the carrier. Alternatively, the anchoring group is covalently attached to the polymer followed by attaching the resultant product to the carrier and then covalently attaching a biomolecule to the polymer. The polymer may be starch, dextran, agarose, polyethylene glycol or polyvinyl alcohol. An attached ligand or binder for the ligand is useful in affinity separations and immunoassays.

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