Immobilization of biomolecules on a fluorocarbon surface with a

Details Immobilization of biomolecules on a fluorocarbon surface with a Immobilization of biomolecules on a fluorocarbon surface with a

1994-10-05

1996-02-13

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 4, 435180, 435803, 435815, 435817, 436532, 530413, 530816, 530813, 536 184, 536 174, 536 179, 536 182, C12N 1106, C12N 1108, C07H 1500, C07K 318

Patent

active

054910838

ABSTRACT:
Poly(fluoroalkyl) sugar reagents are prepared containing a sugar such as a monosaccharide or a disaccharide to which are bonded a plurality of fluoroalkyl anchor groups capable of attaching to a fluorocarbon surface, and either a reactive group capable of covalent coupling to a biomolecule such as an enzyme or a charged group to form an ion-exchanger or a non-ionic group to give a neutral fluorosurfactant. A spacer may be between the reactive group and the sugar. The poly(fluoroalkyl) sugar reagents are strongly adsorbed onto fluorocarbon surfaces to provide supports for such applications as separation and immobilization of biomolecules such as enzymes, carrying out heterogeneous diagnostic assays, and preparation of biosensors.

REFERENCES:
patent: 4954444 (1990-09-01), Eveleigh et al.
patent: 5079155 (1992-01-01), Cox et al.
patent: 5342772 (1994-08-01), Aranzen et al.
De Miguel, et al., Chromatographia, 24:849-853 (1987).

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