Immobilization of biologically active substances with a polyphos

Details Immobilization of biologically active substances with a polyphos Immobilization of biologically active substances with a polyphos

1993-09-16

1995-01-10

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435180, 435803, 435815, 436531, 436532, 528399, 530413, 530815, 530816, C12N 1106, G01N 33549, C08G 7902, C07K 1700

Patent

active

053806581

ABSTRACT:
A phosphazene polymer carrier is prepared that has functional groups capable of binding a biologically active substance such as an enzyme or antibody and groups which are non-reactive and hydrophilic. A bifunctional aldehyde is reacted with primary amino groups of a shaped phosphazene polymer to form side chains having aldehyde groups, an amino group-containing compound is reacted with a portion of the aldehyde groups to produce the groups that are non-reactive and hydrophilic, and aldehyde groups not reacted are capable of binding a biologically active substance. The phosphazene polymer may be crosslinked prior to reacting with the bifunctional aldehyde. In another embodiment, a protecting agent is reacted with a portion of the aldehyde groups to block the aldehyde groups, imino bonds are reduced, a compound having an amino group is reacted with aldehyde groups not blocked to form the groups which are non-reactive and hydrophilic, and the protecting agent is removed to provide aldehyde groups capable of binding a biologically active substance. The non-reactive and hydrophilic groups may also be formed by reacting primary amino groups with formaldehyde or by diazotizing primary amino groups of the polymer followed by hydrolysis to form hydroxyl groups. A biologically active substance immobilized on the polymer can be used to separate a substance that has affinity for the immobilized biologically active substance.

REFERENCES:
patent: 3666733 (1972-05-01), Epton
patent: 3669841 (1972-06-01), Miller
patent: 4440921 (1984-04-01), Allcock et al.
patent: 4451619 (1984-05-01), Heilmann et al.
patent: 5102798 (1992-04-01), Guiseppi et al.
John Wiley & Sons, Encyclopedia of Polymer Science and Engineering, 1988, pp. 31-41, vol. 13.
World Patents Index Latest, Derwent Publications, Ltd., London, GB; AN 89-080047 & JP-A-1 030 650 (Teijin) Feb. 1, 1989.
World Patent Index Latest, Derwent Publications, Ltd., London, GB; AN 85-163620 & SU-A-1 085 993 (Moscow Mendeleev) Apr. 15, 1984.
Macromolecules, vol. 19, No. 6, Jun. 19, 1986, pp. 1502-1508; H. R. Allcock; "Covalent Linkage of Proteins".
Macromolecules, vol. 16, No. 9, 1402-1406, 1983.

Affiliated with

Also associated with

Rating

Immobilization of biologically active substances with a polyphos does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immobilization of biologically active substances with a polyphos, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immobilization of biologically active substances with a polyphos will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-850227