Immobilization of biologically active material in capsules prepa

Details Immobilization of biologically active material in capsules prepa Immobilization of biologically active material in capsules prepa

1989-08-11

1992-05-26

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 702, 435 7021, 435177, 435182, C12N 1110, C12N 1102, C12P 2108

Patent

active

051167476

ABSTRACT:
Biologically-active material, particularly hybridoma cells, is immobilized for mass culture in bioreactors to produce biological products, particularly monoclonal antibodies, by ionically-interacting polycationic groups on chitosan with polyanionic groups on a polyanionic water-soluble polymer. Preferably, the chitosan is chitosan acetate and the polyanionic polymer is sodium alginate. In one embodiment, droplets of a suspension of the biologically-active material and water-soluble polymer are gelled by forming a calcium salt of the water-soluble polymer to form temporary capsules, and a sequestering agent for the calcium ions is added to effect ionotropic gelation of the water-soluble polymer and chitosan to form a semi-permeable membrane around each temporary capsule. In another embodiment, porous beads are formed by exposing droplets of a suspension of the biologically active material and water-soluble polymer to an aqueous solution of chitosan and multiple cations. In a further embodiment, chitosan is added to a suspension of the biologically active material and water-soluble polymer and the resulting suspension is extruded into a solution of multi-valent anions to form a fibrous mass.

REFERENCES:
patent: 4352883 (1982-10-01), Lim
patent: 4749620 (1988-06-01), Rha et al.
patent: 4803168 (1989-02-01), Jarvis, Jr.
patent: 4808707 (1989-02-01), Daly et al.
patent: 4921796 (1990-05-01), Rozzell

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