Immobilization of biological material with an acrylonitrile poly

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-4-aza-bicyclo

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435177, 435180, 435181, 435288, 435 47, 435 49, C12P 3706, C12N 1108, C12N 1106, C12P 3500

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043716121

ABSTRACT:
Biological material such as enzymes is immobilized by adsorption or covalent bonding to a micro-porous water-insoluble acrylonitrile polymer containing from 20 .mu.M to 1000 .mu.M of amino groups per gram of the polymer. Covalent bonding may be carried out with a crosslinking or condensing agent. The amino groups may be introduced into the polymer by reduction of nitrile groups with lithium aluminum hydride in an inert non-solvent.

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patent: 4267273 (1981-05-01), Smith
Inman, et al., The Immobilization of Enzymes on Nylon Structures and Their Use in Automated Analysis, Biochem. J., vol. 129, 1972, (pp. 255-262).
Sundaram et al., Preparation and Properties of Urease Chemically Attached to Nylon Tube, FEBS Letters, vol. 10, No. 5, 1970, (pp. 325-327).
Cohen et al., Reduction of Polymers Using Complex Metal Hydrides., J. Org. Chem., vol. 24, 1959, (pp. 1404-1407).

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