Immobilization of biochemical substances on a carrier containing

Details Immobilization of biochemical substances on a carrier containing Immobilization of biochemical substances on a carrier containing

1993-03-22

1996-02-27

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

435176, 435179, 435181, 436524, 436530, 436532, 530811, 530814, 530816, C12N 1100, C12N 1114, G01N 33551

Patent

active

054948150

ABSTRACT:
Biochemical substances, such as enzymes, are immobilized using an olefinic-unsaturated, epoxyfunctional polysiloxane. The polysiloxane is applied to a carrier material. The polysiloxane on the carrier is cross-linked by using high-energy radiation or a peroxide to form a polymer matrix. The polymer matrix is treated with an aqueous solution of a biochemical substance that reacts with epoxy groups and becomes immobilized. The polymer matrix is stabilized by the reaction of non-reacted epoxy groups with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group. The crosslinked polysiloxane can be hydrophilized after cross-linking and prior to immobilization of the biochemical substance by the reaction of a portion of the epoxy groups with a hydrophilic compound.

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S. A. Barker and I. Kay in "Handbook of Enzyme Biotechnology", (Editor: A. Wiseman), Ellis Horwood, Chichester 1975, Chapter 5, pp. 74-89.

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