Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1982-09-28
1984-03-20
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435177, 435180, 435181, C12P 1920, C12N 1102, C12N 1108, C12N 1106
Patent
active
044381964
ABSTRACT:
Disclosed is a process whereby enzymes are immobilized on activated granular carbon. The process involves treating the carbon with a polyamine compound having pendant amino groups to cause the polyamine to adhere to the carbon leaving pendant amine groups free to further react. The free amine groups are derivatized by treatment with a difunctional compound having amine reactive moieties, so that free amine groups of the enzyme can be covalently bound to the polyamine via the amine reactive compound. By this method, enzymes can be immobilized onto granular carbon which provides a support having excellent physical properties.
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Liu, et al., Biotechnol. Bioeng. 17, 1695-1696, (1975).
Cho, et al., Biotechnol. Bioeng, 20, 1651-1665, (1978).
Jeffers Jerome L.
Miles Laboratories Inc.
Naff David M.
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