Immobilization of biocatalysts on granular carbon

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435177, 435180, 435181, C12P 1920, C12N 1102, C12N 1108, C12N 1106

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active

044381964

ABSTRACT:
Disclosed is a process whereby enzymes are immobilized on activated granular carbon. The process involves treating the carbon with a polyamine compound having pendant amino groups to cause the polyamine to adhere to the carbon leaving pendant amine groups free to further react. The free amine groups are derivatized by treatment with a difunctional compound having amine reactive moieties, so that free amine groups of the enzyme can be covalently bound to the polyamine via the amine reactive compound. By this method, enzymes can be immobilized onto granular carbon which provides a support having excellent physical properties.

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Liu, et al., Biotechnol. Bioeng. 17, 1695-1696, (1975).
Cho, et al., Biotechnol. Bioeng, 20, 1651-1665, (1978).

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