Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-03-10
1998-10-06
Huff, Sheela
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 4, 435 71, 435 791, 435 792, 435 793, 435 794, 435 795, 435174, 435175, 435176, 435177, 435178, 435179, 435180, 435961, 436518, 436523, 436524, 436525, 436526, 436527, 436528, 436529, 436530, 436531, G01N 3353, G01N 33569, G01N 33537, G01N 33543
Patent
active
058174702
ABSTRACT:
This invention is related to a process for the enhanced immobilization of a ligand to solid supports to be used for a biochemical detection method. The enhanced immobilization of the ligand was obtained by coating the surface of the solid support with the adhesive polyphenolic protein isolated from mussels. The bound ligand is reacted with a solution containing an antiligand whereby the antiligand becomes bound to the immobilized ligand. After removing the excess or unbound antiligand, the antiligand bound to the immobilized ligand is detected by using an enzyme-linked immunoassay.
REFERENCES:
patent: 4446231 (1984-05-01), Self
patent: 4585585 (1986-04-01), Waite
patent: 5015677 (1991-05-01), Benedict et al.
patent: 5024933 (1991-06-01), Yang et al.
patent: 5108923 (1992-04-01), Benedict et al.
patent: 5202526 (1993-04-01), Maugh et al.
patent: 5242808 (1993-09-01), Maugh et al.
patent: 5410023 (1995-04-01), Burzio
Sigma Catalog p. 1255, 1993.
Practice and Theory of Enzyme Immunoassays; P. Tijssen 1985, pp. 297-329.
A Method for Attachment of Peptides to a Solid Surface with Enhanced Immunoreactivity; BioTechniques; vol. 13 No. 5 (1992) pp. 739-742.
Nature's Underwater Adhesive Specialist; Int. J. Adhesion and Adhesives; vol. 7 (1987) pp. 9-12.
Marine Bioadhesive: Projections in Medicine and Industry; Current Topics in Marine Biotechology; pp. 353-356 (1989).
Purification of Adhesive Proteins From Mussels; Protein Expression and Purification; 1, 147-150 (1990).
Immunological Studies of the Polyphenolic Proteins of Mussels; Comp. Biochem. Physiol.; vol. 98B. No. 4, pp. 569-572 (1991).
Chimeric IgG-binding Receptors Engineered for Staphylococcal Protein A and Stretococcal Protein G; The Journal of Biological Chemistry; vol. 263, No. 9, Issue of Mar. 25, pp. 4323-4327 (1988).
.alpha.,.beta.-Dehydro-3,4-dihydroxyphenylalanine Derivatives; Potential Schlerotization Intermediates in Natural Composite Materials; Archives of Biochemistry and Biophysics; vol. 285, No. 1, Feb. 15, pp. 17-26, (1991).
Clinical Chemistry, vol. 36, No. 8, 1990, "A Decade of Development in Immunoassay Methodology", pp. 1408-1426.
Clinical Chemistry, vol. 40, No. 3, 1994, "Selected Strategies for Improving Sensitivity and Reliability . . . ", pp. 347-357.
Arch. Biol. Med. Exp 23, Burzio et al, "Bioadhesives: A biotechnological opportunity", pp. 173-178, 1990.
1994 Aldrich Chemical Company, Inc., "Chemists helping chemists", ordering information, p. 1271.
Burzio Luis O.
Burzio Veronica A.
Huff Sheela
Sociedad Biotecnologica Collico Limitada
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