Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1990-12-06
1992-04-21
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435180, 435182, 423366, 536 28, 558232, 564 17, C12N 1110, C12N 1108, C12N 1104, C07H 1700
Patent
active
051067404
ABSTRACT:
A cofactor having an aromatic or benzylic amino group is converted into an isothiocyanate by reaction with a compound such as thiophosgene and the isothiocyanate of the cofactor is attached to a polymer which is preferably water-soluble. When the polymer has amino groups, a thiourea bridge is formed between the polymer and cofactor and a polymer having hydroxyl groups results in a thiocarbamate bridge. The polymer may be a copolymer of vinylamine or vinylmethylamine and vinylmethylacetamide, partially alkylamine-substituted .alpha., .beta.-poly-(2-hydroxyethyl)-D,L-aspartamide, polyethyleneimine or carbohydrate. The cofactor bound to a polymer and an enzyme are contained in microcapsules or in a membrane to form an enzyme reactor.
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Jacob Grunwald et al., Nylon Polyethyleneimine Microcapsules for Immobilizing Multienzymes with Soluble Dextran-Nad.sup.+ for the Continuous Recycling of the Microencapsulated Dectran-Nad.sup.+, Biochem. and Biophys. Res. Comm., vol. 81, No. 2, (1978) pp. 565-570.
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Bader Hubert
Hoppe Hans-Ullrich
Magerstadt Michael
Schlingmann Merten
Ulschneider Dieter
Hoechst Aktiengesellschaft
Naff David M.
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