Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1978-03-14
1980-08-12
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 22, 435 99, 435176, 435178, C07G 702, G01N 3114, G01N 3108, C12D 1304
Patent
active
042174155
ABSTRACT:
A carbohydrate substrate such as starch for a carbohydrate hydrolyzing enzyme is immobilized on a solid inorganic porous support to form a stable substrate-support composite useful in affinity chromatography and in methods where a precise amount of substrate is needed to perform an enzyme-substrate reaction to quantify the enzyme. The substrate may be activated with an agent such as cyanogen bromide or imidazole prior to deposition on the support so that it may be effectively modified while on the support. After deposition, the substrate is modified by reaction with an epoxyhalogen, aliphatic dihalide or aliphatic diamine to aid in holding it on the support. In an alternative embodiment, the carbohydrate, prior to deposition and modification on the support, is hydrolyzed with an enzyme, preferably dextranase.
REFERENCES:
patent: 2459108 (1949-01-01), Lolkema
patent: 2626257 (1953-01-01), Caldwell et al.
patent: 3208918 (1965-09-01), Beers, Jr.
patent: 3251949 (1966-05-01), Lipps, Jr.
patent: 3436309 (1969-04-01), Ottinger et al.
patent: 3912590 (1975-10-01), Slott et al.
patent: 3918183 (1975-10-01), Johansson et al.
patent: 3996107 (1976-12-01), Martensson
patent: 4033820 (1977-07-01), Brouillard
patent: 4043871 (1977-08-01), Blixt et al.
patent: 4048018 (1977-09-01), Coughlin et al.
Guilbault et al., Assay of Cholinesterase in an Electrode Systems with an Immobilized Substrate, Analytica Chim. Acta., vol. 85, 1976, pp. 295-306).
Barabino Raymond C.
Keyes Melvin H.
Naff David M.
Technicon Instruments Corporation
Tedesco S. P.
LandOfFree
Immobilization of an enzyme substrate does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immobilization of an enzyme substrate, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immobilization of an enzyme substrate will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2182972