Immobilization of active protein by cross-linking to inactive pr

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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260112R, 260121, 435179, 435181, 435182, C12N 1102, C12N 1106, C07G 700

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active

044644680

ABSTRACT:
A solution of an active protein substance and an inactive protein substance is reacted with a cross-linking agent, optionally in the presence of an inert carrier, under cross-linking conditions to produce articles comprising both active and inactive protein substances. The active protein substance comprises up to about 20 percent, e.g. from 1 to 20 percent by weight, based on the final weight of the total protein substance, whereas the cross-linking agent comprises from 0.5 to 8 percent by weight, based on the weight of the total treated mixture. The obtained articles are in the form of a solution or a suspension in aqueous medium, in the form of a film, in the form of a membrane, in the form of a fabric, in the form of a porous material, or in the form of a mass, such as granules, pills or tablets.

REFERENCES:
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patent: 4004979 (1977-01-01), Avrameas et al.
Silman et al., Some Water-Insoluble Papain Derivatives Biopolymers, vol. 4, 1966, (pp. 441-448).
Avrameas et al., Biologically Active Water-Insoluble Protein Polymers, Journal of Biological Chemistry, vol. 242, No. 7, 1967, (pp. 1651-1659).
Habeeb A. F. S. A., Preparation of Enzymically Active Water-Insoluble Derivatives of Trypsin, Archives of Biochemistry and Biophysics, vol. 119, 1967, (pp. 264-268).
Sabatini et al., The Journal of Cell Biology, vol. 17, pp. 19-58, 1963.
Schick et al., The Journal of Biological Chemistry, vol. 236, pp. 2477-2485, 1961.

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