Immobilization of active agents on substrates with a silane and

Details Immobilization of active agents on substrates with a silane and Immobilization of active agents on substrates with a silane and

1989-01-13

1991-12-31

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an...

435181, 436524, 436527, 436532, 530811, 530816, C12N 1114, C12N 1104, G01N 33551, G01N 33549

Patent

active

050772104

ABSTRACT:
Active agents such as proteins are covalently immobilized on substrates carrying hydroxyl groups. A silane is bound to the substrate and coupled to a heterobifunctional crosslinker at one functional group leaving a free functional group, different than the first group, to which a protein is bound while retaining high protein functionality. Preferably, the silane has a functional group which reacts with the hydroxyl group of the substrate and a thiol terminal group which reacts with a functional group of a heterobifunctional crosslinking agent which contains a succinimide group that reacts with an amino group of the active agent. Bound active agents such as proteins are useful as biosensors or reactants in a variety of applications including bioassays.

REFERENCES:
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patent: 4517303 (1985-05-01), Freytag et al.
patent: 4894229 (1990-01-01), Polson et al.
Weetall, H. H., Methods in Enzymology, vol. 44, 1976, Academic Press, N.Y., pp. 134-149.
Jonsson et al., Method in Enzymology, vol. 137, 1988, Academic Press, N.Y., pp. 381-394.

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