Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1989-01-30
1993-11-02
Lacey, David L.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530413, 435 697, 4353201, 43525421, 536 234, 536 2374, 935 69, C07K 318, C07K 1504, C12P 2100, C12N 1556
Patent
active
052585026
ABSTRACT:
A gene fusion construction encoding a protein of interest and the chitin binding domain of a chitinase enzyme and the encoded fusion protein, which is the protein of interest and a protein capable of binding chitin. A plasmid vector containing the gene fusion construction is also described. The vector is used to transform host cells, which produce and secrete the recombinant fusion protein.
The fusion protein is isolated by binding to chitin through its chitin-binding domain. The present method uses chitin to bind the fusion protein through its chitin-binding domain, and thereby allows immobilization and/or purification of the protein of interest, using known techniques.
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Elliott George C.
Lacey David L.
Massachusetts Institute of Technology
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