Immersion microscope objective and laser scanning microscope...

Optical: systems and elements – Lens – Microscope objective

Reexamination Certificate

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C359S656000, C359S368000

Reexamination Certificate

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07869132

ABSTRACT:
An immersion microscope objective formed of thirteen or fewer lens elements includes, in order from the object side, first and second lens groups of positive refractive power, a third lens group, a fourth lens group having negative refractive power with its image-side surface being concave, and a fifth lens group having positive refractive power with its object-side surface being concave. The first lens group includes, in order from the object side, a lens component that consists of a lens element of positive refractive power (when computed as being in air) and a meniscus lens element having its concave surface on the object side. Various conditions are satisfied to ensure that images of fluorescence, obtained when the immersion microscope objective is used in a laser scanning microscope that employs multiphoton excitation to observe a specimen, are bright and of high resolution. Various laser scanning microscopes are also disclosed.

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English translation of pp. 80-82 of “Introduction to Optics”, first edition, eighth printing, published in Japanese by The Optronics Co., Ltd. on Mar. 29, 2002.
English translation of pp. (171)41-(175)45 of Eiji Yokoi et al., “Industrial Info.—A new water immersion objective lens specifically designed for two-photon imaging”, published in SAIBOU (The CELL) by New Science Co., Ltd. on Apr. 20, 2008.

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