Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1983-02-22
1986-02-04
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
436537, 436800, 436804, 436815, 436817, 436824, G01N 3352, G01N 3354, G01N 3358
Patent
active
045686491
ABSTRACT:
A test kit and assay for detecting the presence of minute amounts of an organic reactant in a test sample includes a plurality of beads or other types of support structures that are impregnated with a fluorescer and coated with a ligand that specifically binds to the organic reactant being investigated. The beads are placed in an aqueous solution, together with the reactant which has been radiolabeled. The portion of the radiolabeled reactant that binds to the ligand is thereby brought in close enough proximity to the beads to activate the fluorescer to produce light energy. The radiolabeled reactant that does not bind to the ligand is, for the most part, disposed too far away from the beads to enable the radioactive energy emitted thereby to reach the fluorescer integrated into the beads. Thus, the level of light energy produced by the fluorescer is indicative of the amount of reactant present in the test sample.
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Hart and Greenwald, "Scintillation Proximity Assay (SPA)--A New Method of Immunoassay", Molecular Immunology, 265 (1979).
Hart and Greenwald, "Scintillation-Proximity Assay of Antigen-Antibody Binding Kinetics: Concice Communication", 20, Journal of Nuclear Medicine, 1062 (1979).
Immunex Corporation
Nucker Christine M.
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