IL-1.beta.protein precursor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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424 852, 930141, 435 6952, C07K 14545

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active

055104622

ABSTRACT:
The subject invention concerns a nucleic acid comprising a nucleotide sequence encoding human interleukin-1 (IL-1), and fragments thereof, and the polypeptides and peptides obtained. Specifically, the subject invention comprises the cloning of a cDNA synthesized by reverse transcription of poly(A)RNA isolated from adherent human monocytes-stimulated with bacterial endotoxin. Human IL-1 is useful to induce the production of IL-2 by activated T-cells; it also acts on B-cells and NK-cells.

REFERENCES:
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Dinarello, C.A. (1984) "Interleukin-1" Reviews of Infectious Diseases, 6: 51-95.
Roesenwasser, L. J., Dinarello, C.A., and Rosenthal, A. S. (1979) "Adherent cell function in murine T-lymphocyte antigen recognition" J. Exp. Med. 150: 709-714.
Rosenwasser, L. J. and Dinarello, C. A. (1981) "Ability of human leukocytic pyrogen to enchance phytohemegglutinin induced murine thymocyte proliferation" Cellular Immunology 63: 134-142.
Murphy, P. A., Simon, P. L., and Willoughby, W. F. (1980) "Endogenous pyrogens made by rabbit peritoneal exudate cells are identical with lymphocyte-activating factors made by rabbit alveolar macrophages" J. Immunol. 124: 2498-2501.
Kampschmidt, R. F. (1981) "leukocytic endogenous mediator/endogenous pyrogen" The Physiologic and Metacolic Responses of the Host (eds M. C. Powanda and P. G. Canonico) pp. 55-74 (Elservier/North-Holland, Amsterdam.
Mizel, S. B., Dayer, J. M., Krane, S. M., and Mergenhagen, S. E. (1979) "Stimulation of Rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin-1)" Proc. Natl. Acad. Sci. USA 78: 2474-2477.
D. J. Hazuda et al., J. Biol. Chem. 265(11): 6318-6322, Apr. 15, 1990.
B. Mosley et al., Proc. Nat. Acad. Sci. 84: 4572-4576, Jul. 1987.
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