IgG depleted serum preparations and methods for antibody product

Details IgG depleted serum preparations and methods for antibody product IgG depleted serum preparations and methods for antibody product

1993-12-07

1997-01-14

Wityshyn, Micheal G.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435 701, 435 702, 435 7021, 4352402, 43524026, 43524027, 4352403, A01N 102, C12P 2104, C12N 500

Patent

active

055938221

ABSTRACT:
A method for preparing serum depleted of IgG to be used in the production of IgG monoclonal antibodies is disclosed. Animal serum is contacted with protein G or protein A prior to addition to base media to obtain a superior cell culture medium depleted of serum derived IgG antibodies. Included in the disclosure are improved media preparations for cell culture of antibody producing cells and incorporating the antibody depleted serum and improved methods of producing the antibodies.

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