Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1995-04-14
1997-07-22
Jagannathan, Vasu S.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530399, 530408, 530412, 435 694, C07K 14475, C12N 1518
Patent
active
056504969
ABSTRACT:
Disclosed is an improved process for obtaining purified, monomeric, intact, correctly-folded insulin-like growth factor-I (also known as somatomedin-C). The improvements, consisting primarily of the addition of an IGF-I unfolding/refolding step and the substitution of a reverse phase chromatography step for a gel filtration chromatography step result in a three-fold increase in final yield. The process includes the following steps, in order: first cation exchange, unfolding/refolding, hydrophobic interaction chromatography, second cation exchange, and reverse phase chromatography.
REFERENCES:
patent: 5093317 (1992-03-01), Lewis et al.
patent: 5231178 (1993-07-01), Holtz et al.
patent: 5288931 (1994-02-01), Chang et al.
patent: 5324639 (1994-06-01), Brierley et al.
patent: 5407810 (1995-04-01), Builder et al.
patent: 5410026 (1995-04-01), Chang et al.
patent: 5446024 (1995-08-01), Builder et al.
patent: 5451660 (1995-09-01), Builder et al.
patent: 5459052 (1995-10-01), Skriver et al.
Axellson et al., "Disulfide arrangement of human insulin-like growth factor I derived from yeast and plasma," J. Biochem. 206:987-994 (1992).
Bayne et al., "Expression, purification and characterization of recombinant human insulin-like growth factor I in yeast," Gene 66:235-244 (1988).
Browning et al., "Disulfide Scrambling of Interleukin-2: HPLC Resolution of the Three Possible Isomers," Analytical Biochemistry, 155:123-128 (1986).
Chang et al., "Single-Step Solubilization and Folding of IGF-1 Aggregates from Escherichia coli, " Chapter 14, in Protein Folding In Vivo and In Vitro, Am. Chem. Soc., pp. 178-188 (1993).
Chang et al., "Folding of IGF-I," BIOT, Abstract #117.
Hart et al., "Effect of environment on insulin-like growth factor I refolding selectivity," Biotechnol. Appl. Biochem 20:217-232 (1994).
Hart et al., "Large Scale, In Situ Isolation of Periplasmic IGF-I from E. coli, " Bio/Technology 12:1113-1117 (1994).
Hejnaes et al., "Development of an optimized refolding process for recombinant Ala-Glu-IGF-1," Protein Engineering, 5:797-806 (1992).
Hober et al., "Disulfide Exchange Folding of Insulin-like Growth Factor I," Biochemistry 31:1749-1756 (1992).
Meng et al., "Reduction Studies on Bacterial Recombinant Somatomedin C Insulin-Like Growth Factor-1," Journal of Chromatography 443:183-192 (1988).
Miller et al., "Oxidative Refolding of Insulin-like Growth Factor 1 Yields Two Products of Similar Thermodynamic Stability: A Bifurcating Protein-Folding Pathway," Biochemistry 32:5203-5213 (1993).
Niwa et al., "Chemical Synthesis, Cloning, and Expression of Genes for Human Somatomedin C (Insulin-like Growth Factor I) and .sup.59 Val-Somatomedin C," Ann. NY Acad. Sci. 469:31-52 (1986).
Olson et al., "Preparative isolation of recombinant human insulin-like growth factor 1 by reversed-phase high-performance liquid chromatography," Journal of Chromatography A, 675:101-112 (1994).
Petrides et al., "An Improved Method for the Purification of Human Insulin-Like Growth Factors I and II," Endocrinology 118:2034-2038 (1986).
Pfeifle et al., "Insulin-Like Factor 1/Somatomedin-C: A Rapid Isolation Procedure With FPLC," Preparative Biochemistry 15:291-307 (1985).
Scrip No. 910, p. 21 (1984).
Steiner et al., "The Spontaneous Reoxidation of Reduced Beef and Rat Proinsulins," Biochemistry 60:622-629 (1968).
Sofer et al. BioTechniques. Nov./Dec.: 198-203, 1983.
Abrams Joan N.
Brierley Russell A.
Hanson John M.
Maslanka Francis C.
Cephalon Inc.
Jagannathan Vasu S.
Saoud Christine
LandOfFree
IGF-I purification process does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with IGF-I purification process, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and IGF-I purification process will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1560649