Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-02-06
2007-02-06
Weber, Jon (Department: 1653)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
Reexamination Certificate
active
10783795
ABSTRACT:
Methods for identifying and characterizing inhibitors of protein filament formation are provided. These methods are particularly useful for identifying those agents which inhibit or prevent protein filament formation within neurons of mammalian subjects, particularly human subjects, such as the formation of tau filaments in Alzheimer's patients, and α-synuclein filaments in Parkinson's patients. According to the methods, protein monomers which are associated with formation of intra- or extra-cellular aggregates are combined under physiological conditions with a fibrillization inducer and the formation of protein aggregates is assessed in the absence and the presence of a test agent. The absence or a reduction in the size or stability of proteinaceous polymeric filaments as compared to a control indicates that the test agent is an inhibitor of proteinaceous polymeric filament formation.
REFERENCES:
patent: 6177472 (2001-01-01), Wilson et al.
patent: 6458847 (2002-10-01), Wilson et al.
patent: 6479528 (2002-11-01), Kuret et al.
patent: 2002/0151464 (2002-10-01), Wolozin et al.
patent: 2002/0152480 (2002-10-01), Biere et al.
patent: 2002/0164657 (2002-11-01), Sharma et al.
patent: 2002/0168687 (2002-11-01), Wischik et al.
patent: 2002/0197737 (2002-12-01), Mandelkow et al.
Cohlberg et al., “Heparin and Other Glycosaminoglycans Stimulate the Formation of Amyloid Fibrils from alpha-Synuclein in Vitro”. Biochemistry. 2002. vol. 41, pp. 1502-1511.
Definition of Surfactant from Wikipedia: (http://en.wikipedia.org.wiki.Surfactant), 2005.
International search report for PCT application No. PCT/US2004/005097.
King, et al., Differential Assembly of Human Tau Insoforms in the Presence of Arachidonic Acid J. Neurochem., 74, 1749-1757, 2000.
Tabaton, et al, Ultrastructural localization of beta-amyloid, tau and ubiquitin eptiopes in extracellular neurfibrillary, Proc Nat Acad Sci, vol. 88, p. 2098-2102, Mar. 1991.
Lansbury, Jr., Fibrillogenesis and Neurodegenerative Diseases. What is the Link?, Natl. Acad. Sci. USA vol. 96, pp. 3342-3344, Mar. 1999.
Pompe-Novak, et al., Neural inclusions in the light of new findings on protein fibrillization, Cognitive Neuroscience, Information Society 2001, pp. 72-75.
Caughey, et al., Protofibrils, Pores, Fibrils, and Neurodegeneration: Separating the Responsible Protein Aggregates from the Innocent Bystanders, Annu. Rv. Neurosci. 2003.
Necula, et al., Rapid anionic Micelle-mediated Synuclein Fibrillization in Vitro, The Journal of Biological Chemistry, vol. 278, Nov. 21, pp. 46674-46680, 2003.
Chirita, et al., Anionic Micelles and Vesicles Induce Tau Fibrillization in Vitro, The Journal of Biological Chemistry, vol. 278, No. 28, Jul. 11, pp. 25644-25650, 2003.
Alonso, et al., Hyperphosphorylation indcues self-assembly of into tangles of paired helical filaments/straight filaments, PNAS, vol. 98, No. 12, Jun. 5, 2001, pp. 6923-6928.
Sontag, et al., Molecular Interactions Among Protein Phosphatase 2A, Tau, and Microtubules, Journal of Biological Chem., vol. 274, No. 36, pp. 25490-25498, Sep. 3, 1999.
Crystal, et al., A comparison of amyloid fibrillogenesis using the novel fluorescent compound K114, Journal of Neurochemistry, 86, pp. 1359-1368, 2003.
Forman, et al., Convergence of Tau and Synuclein Pathology in Neurodegenerative Disease, American Society for Investigative Pathology, 2003.
Rochet, et al., Inhibition of fibrillization and accumulation of prefibrillar oligomers in mixtures of human and mouse alpha-synuclein, Biochem., 39(35):10619-26, Sep. 5, 2000.
Chitra, et al., Ligand-Dependent Inhibition and Reversal of Tau Filament, Biochemistry 2004, 43, 2879-2887.
Necula, et al., Electron microscopy as a quantative method for investigating tau fibrillization, Analytical Biochemistry 329 (2004) 238-246.
Necula, et al., A static laser light scattering assay for surfactant-induced tau fibrillization, Analytical Biochemistry 333 (2004) 205-215.
Necula, et al., Pseudophosphorylation and Glycation of Tau Protein Ehnance but Do Not Trigger Fibrillization in Vitro, The Journal of Biological Chemistry, vol. 279, No. 48, Nov. 26, pp. 49694-49703, 2004.
Necula, et al., Site-specific pseudophosphorylation modulates the rate of tau filament dissociation,FEBS Letters 579 (2005) 1435-1457.
Kuret, et al., Pathways of tau fibrillization, Biochimica et Biophysica Acta 1739 (2005) 167-178.
Guibin, et al., Casein Kinase 1 Delta Phosphorylates Tau and Disrupts Its Binding to Microtubules, The Journal of Biochemical Chemistry, vol. 279, No. 16, Apr. 16, pp. 15938-15945, 2004.
Chirita et al. Triggers of Full-Length Tau Aggregation: A Role for Partially Folded Intermediates, Biochemistry 44 5862-5872 2005.
Chirita, et al., Evidence for an Intermediate in Tau Filament Formation, Biochemistry 43, 1704-1714 2004.
Chirita Carmen N.
Kuret Jeff
Necula Mihaela
Calfee Halter & Griswold LLP
Noakes Suzanne M.
The Ohio State University Research Foundation
Weber Jon
LandOfFree
Identifying inhibitors of intracellular protein fibrillization does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Identifying inhibitors of intracellular protein fibrillization, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Identifying inhibitors of intracellular protein fibrillization will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3866407