Identification test for highly refined sesame oil

Chemistry: analytical and immunological testing – Including chromatography

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436174, 436177, 436178, 252398, 424439, 426330, 4263306, 426542, 426545, 426603, 426605, 426606, 426607, 426611, 426615, 426634, 549386, 549435, 549464, G01N 3002, G01N 100, G01N 118

Patent

active

058113134

ABSTRACT:
A method for confirming the presence of highly refined sesame oil in pharmaceuticals. By extracting, concentrating and detecting sesamin and epi-sesamin, through the method disclosed in the present invention, highly refined sesame oil can be identified. The disclosed method can be performed at room temperature and does not require over night extraction. In practice, the disclosed method entails extracting sesamin from a sample with an organic solvent, transferring and evaporating the solvent layer to dryness to form a residue. An alkyl halide is added to the residue to form a mixture. The mixture and an organic solvent are passed through a separating means, generating an eluent, the eluent is then evaporated to dryness to form a second residue. A 1:1 solution of an organic solvent and an alkyl halide is added to the second residue in a volume sufficient to form a concentrated extract. The concentrated extract is then analyzed with high performance liquid chromatography.

REFERENCES:
patent: 4708820 (1987-11-01), Namiki et al.
patent: 5180588 (1993-01-01), Shinmen et al.
patent: 5209826 (1993-05-01), Ozaki et al.
patent: 5211953 (1993-05-01), Shinmen et al.
patent: 5336496 (1994-08-01), Akimoto et al.

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