Identification of plant-responsive genes of bacteria

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 691, 435 698, 435 711, 4351721, 4351723, 435243, 435244, 4352533, 4353201, 435 911, 4352523, 536 231, 536 237, 935 19, 935 77, 935 78, 935 88, C12Q 168, C07H 2104, C12N 121, C12P 2100

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053976975

ABSTRACT:
The invention is a method for identifying and cloning bacterial genes that are responsive to the presence of a plant host, comprising mutating a bacterial culture with a transposon containing a promoterless marker gene, exposing the bacterial mutants to root exudates, other plant-derived substances or plants, and identifying mutants that have a transposon-associated gene inducible by the plant. These mutants may be used to clone and characterize the promoters and the associated wild-type bacterial genes responsive to the plant.

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