Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2006-05-30
2006-05-30
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S004000, C435S006120, C536S002000
Reexamination Certificate
active
07052865
ABSTRACT:
A method for identifying a gene having a role in the presentation of diabetic nephropathy comprises culturing mesangial cells in the presence of a concentration of glucose sufficient to induce differential expression, especially up-regulation, of a gene susceptible to such differential expression and identifying the gene so induced. The cells are also optionally subjected to mechanical strain and/or TGF-β1 can be added to the culture medium. The differentially expressed genes can be identified by suppression subtractive hybridisation. The method has resulted in the Identification of novel genes which play a role in the presentation of diabetic nephropathy. The genes can be used as diagnostic markers for diabetic nephropathy and as the basis of drug development programmes.
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Brady Hugh Redmond
Godson Catherine Mary
Martin Finian Mary
McMahon Ruth Anne
Murphy Madeline Anne
Birch & Stewart Kolasch & Birch, LLP
Guzo David
Hibergen Limited
Sullivan Daniel M.
University College Dublin, National University of Ireland, Dubli
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