Identification of genes

Details Identification of genes Identification of genes

2006-01-10

2006-01-10

Leffers, Gerry (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S029000, C435S320100, C435S235100, C435S252300, C435S325000, C435S091100, C435S440000, C536S023100

Reexamination Certificate

active

06984490

ABSTRACT:
A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of:(1) providing a plurality of microorganisms each of which is independently mutated by the insertional inactivation of a gene with a nucleic acid comprising a unique marker sequence so that each mutant contains a different marker sequence, or clones of the said microorganism;(2) providing individually a stored sample of each mutant produced by step (1) and providing individually stored nucleic acid comprising the unique marker sequence from each individual mutant;(3) introducing a plurality of mutants produced by step (1) into the said particular environment and allowing those microorganisms which are able to do so to grow in the said environment;(4) retrieving microorganisms from the said environment or a selected part thereof and isolating the nucleic acid from the retrieved microorganisms;(5) comparing any marker sequences in the nucleic acid isolated in step (4) to the unique marker sequence of each individual mutant stored as in step (2); and(6) selecting an individual mutant which does not contain any of the marker sequences as isolated in step (4).

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