Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-11-01
1998-10-20
Low, Christopher S.F.
Chemistry: molecular biology and microbiology
Vector, per se
435194, 4351723, 4353201, 435325, 5367235, C12N 1500
Patent
active
058245456
ABSTRACT:
A polynucleotide encoding chitin synthase (CHS1), an enzyme essential for cell wall synthesis and yeast cell growth, is provided. A maltose responsive promoter (MRP) isolated using the promoter library of the invention is also described.
The present invention also provides a vector for isolation of a eukaryotic regulatory polynucleotide, i.e., promoter. The vector is useful in the method of the invention which comprises identifying a eukaryotic regulatory polynucleotide, i.e., promoter region, by complementing the growth of an auxotrophic host cell containing the vector of the invention, which includes a promoter region operably linked to a promoterless auxotrophic gene. The vector is introduced into the host cell chromosome by targeted integration. Also provided is a library containing host cells having the vector of the invention integrated in the chromosome of the host cell.
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Bulawa Chris
Gavrias Vicky
Koltin Yigal
Riggle Perry
Winter Ken
Low Christopher S.F.
Millennium Pharaceuticals, Inc.
Nguyen Dave Trong
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