Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1998-10-13
1999-11-02
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 4352521, 4352528, 4352534, 4352544, 4352551, 435440, C12Q 104, C12N 114, C12N 120, C12N 1501
Patent
active
059768282
ABSTRACT:
A method for identifying a strain having a conditional lethal mutation which is essential for survival when strain is incubated under restrictive growth conditions, and methods of identifying gene products and gene functions thereof.
REFERENCES:
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patent: 5821076 (1998-10-01), Timberlake et al.
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Leidich et al., "Temperature-sensitive Yeast GPI Anchoring Mutants gpi2 and gpi3 Are Defective in the Synthesis of -Acetylglucosaminyl phosphatidylinositol," J. Biol. Chem., 270:13029-13035, 1995.
Lodge et al., "Comparison of Myristoyl-CoA:Protein N-Myristoyltransferases from Three Pathogenic Fungi: Cryptococcus neoformans, Histoplasma capsulatum, and Candida albicans," J. Biol. Chem, 28:2996-3009, 1994.
Schmid et al., "Genetic Analysis of Temperature-Sensitive Lethal Mutants of Salmonella typhimurium," Genetics, 123:625-633, 1989.
Smith et al., "Hyphal Tip Extension in Aspergillus nidulans Requires the manA Gene Which Encodes Phosphomannose Isomerase," Mol. and Cell. Biol., 14:6030-6038, 1994.
van Zyl et al., "A General Screen for Mutants of Saccharomyces cerevisiae Deficient in tRNA Biosynthesis," Genetics, 123:55-68, 1989.
Winston, "Mutagenesis of Yeast Cells," Current Protocols in Molecular Biology, Supplement 19, 2:13.3.1-13.3.4, 1992.
Bender et al. Use of a Screen for Synthetic Lethal and Multicopy Suppressor Mutants to Identify Two New Genes involved in Morphogenesis in Saccharomyces cerivisiae. Mol. Cell. Biol. 11: 1295-1305, 1991.
Costigan et al. NHP6A and NHP6B, Which Encode HMG-1 Like Proteins, Are Candidates for Downstream Components of the Yeast SLT2 Mitogen-Activated protein Kinase Pathway. Mol. Cell. Biol. 14: 2391-2403, 1994.
Denison et al. Mutation in the bimD gene of Aspergillus nidulans confers a conditional mitotic block and sensitivity to DNA Damaging Agents. Genetics. 134: 1085-1096, 1993.
Gietz et al. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 20(6): 1425, 1992.
Harris et al. Molecular Analysis of Saccharomyces cerevisiae chromosome I on the number of Genes and the Identification of Essential genes using termperature sensitive lethal mutations. J. Mol. Biol. 225: 53-65, 1992.
Hooke. Temperature sensitive mutants of bacterial pathogens: Isolation and use to determine host clearance and in vivo replication rates. Meth. Enzymol. 235: 4458-4457, 1994.
Hou et al. In vivo selection of conditional lethal mutations in the Gene Encoding elongation factor G of Escherichia coli. J. Bacteriol. 176: 123-129, 1994.
Inoko et al. Isolation and characterization of conditional lethal rho mutants of Escherichia coli. PNAS. 74: 1162-1166, 1977.
Karrow et al. The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators. Mol. Microbiol. 7(1): 69-79, 1993.
Leidich et al. Temperature sensitive Yeast GPI Anchoring mutants gpi2 and gpi3 are defective in the synthesis of acetylglycosaminyl phosphatidylinositol. J. Biol. Chem. 270: 13029-13035, 1995.
Lodge et al. Comparison of myristoryl-CoA: Protein N-Myristoyltransferases from three pathogenic fungi: Cryptococcus neoformans, Histoplasma capsulatum and Candida albicans. J. Biol Chem. 28: 2996-3009, 1994.
Schmid et al. Genetic analysis of temperature sensitive lethal mutants of Salmonella typhimurium. Genetics. 123: 625-633, 1989.
Smith et al. Hyphal Tip extension in Aspergillus nidulans requires the manA gene which encodes phosphomannose isomerase. Mol. Cell. Biol. 14:6030-6038, 1994.
van Zyl et al. A general screen for mutants of Saccharomyces cerevisiae deficient in tRNA biosynthesis. Genetics. 123: 55-68, 1989.
Winston. Mutagenesis of yeast cells. Curr. Protocol. in Mol. Biol. Suppl. 9, 2:13.3.1-13.3.4, 1992.
Gavrias Victoria
Timberlake William
Degen Nancy
Millennium Pharmaceuticals Inc.
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